Vaccines have proven to be one of the most effective strategies in controlling infectious diseases and are a key component towards the protection of our health and economy. However, the success of vaccination programs often face considerable challenges in resource-limited regions, primarily due to the necessity of maintaining cold chain throughout transportation and storage. The demand for temperature-stable vaccines has spurred research into innovative strategies for preserving vaccine efficacy under harsh environmental conditions for equitable distribution without the dependence of ‘cold chain’ logistics. Here, we report biomimetic-mineralization of a live viral vaccine using metal organic frameworks (MOFs) to enhance their storage stability. Using Newcastle disease virus vaccine NDV V4 as our candidate, we investigate the impact of different molar concentrations of MOF composites on encapsulation, reporting that an increase in molar concentrations influences recovery of virus. We describe the impacts of lyophilisation on MOF structural integrity, viral titre, and report on alternative techniques such as air drying or column drying, evaluating these strategies with a storage experiment at ambient temperatures. Additionally, we describe using a haemagglutination assay as a quick and easy method to measure MOF encapsulation of NDV, as well as report on a new cell line used in a tissue culture infectious dose assay (TCID50) that can accurately displays cytopathic effect of NDV without immunofluorescent staining.